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利用12对微卫星引物对红鳍笛鲷(♀)、千年笛鲷(♂)及其红鳍笛鲷(♀)×千年笛鲷(♂)子一代(F1)3个群体进行扩增,对3个群体的等位基因频率、群体多态信息含量、有效等位基因数、杂合度、Shannon信息指数及群体间遗传距离进行了遗传检测,共检测到29个等位基因,平均每个基因座位2.4167个等位基因。红鳍笛鲷、F1、千年笛鲷3个群体的多态信息含量分别为0.3484、0.5008、0.4097;有效等位基因数分别为1.9787、2.5074、2.1334;杂合度分别为0.6548、1.0000、0.7384;Shannon信息指数分别为0.6207、0.9240、0.6957。红鳍笛鲷(♀)-F1、F1-千年笛鲷(♂)、红鳍笛鲷(♀)-千年笛鲷(♂)的遗传距离分别为0.3116、0.2346、0.7813。多态信息含量、有效等位基因数、杂合度、Shannon信息指数均显示F1代有杂种优势。
Twelve pairs of microsatellite primers were used to amplify the three populations of red snapper (♀), Millennium snapper (♂) and their red snapper (♀) × Millennium snapper (♂) The alleles, population polymorphism information content, effective allele number, heterozygosity, Shannon’s information index and inter-population genetic distance of three populations were detected by genetic testing. A total of 29 alleles were detected, with the average of each gene Seat 2.4167 alleles. The results showed that the polymorphic information content of three populations of red snapper, F1 and millennium snapper were 0.3484, 0.5008 and 0.4097, respectively. The number of effective alleles was 1.9787, 2.5074 and 2.1334, respectively. The heterozygosities were 0.6548, 1.0000 and 0.7384, respectively. Shannon Information index were 0.6207,0.9240,0.6957. The genetic distances of red snapper (♀) -F1, F1-Millennium snapper (♂), red snapper (♀) -Midan snapper (♂) were 0.3116, 0.2346 and 0.7813, respectively. Polymorphism information content, number of effective alleles, heterozygosity and Shannon’s information index all showed F1 hybrids had heterosis.