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本文涉及无载体酶稳定化问题。介绍无载体酶稳定化新方法——交联酶晶体（Ｃｒｏｓ－ＬｉｎｋｅｄＥｎｚｙｍｅＣｒｙｓｔａｌｓ，ＣＬＥＣｓ），它是近年发展起来的新型酶晶体催化剂，具有酶的一般特性，催化活性和选择性高、在温和条件下反应等，又具有非均相化学催化剂的操作稳定性高、易回收利用的特点。ＣＬＥＣｓ技术是将酶结晶技术和化学交联技术结合，提高酶抵抗极端条件以及有机溶剂中的稳定性，并广泛适应于各种各样的蛋白质及其他领域，如酶传感器、化妆品和洗涤剂等需要高稳定性和高活性蛋白质的领域。
This article deals with the problem of carrier-less enzyme stabilization. A new method of immobilizing enzyme-free immobilized enzyme (Cros-Linked Enzyme Crystals, CLECs) is introduced, which is a new type of enzyme crystal catalyst developed in recent years. It has the general characteristics of the enzyme, high catalytic activity and selectivity. Under mild conditions Reaction, etc., but also has a high chemical stability of the heterogeneous catalyst, easy to recycle features. CLECs technology combines enzyme crystallization with chemical cross-linking to improve enzyme resistance to extreme conditions and stability in organic solvents and is widely used in a wide range of proteins and other fields such as enzyme sensors, cosmetics and detergents Areas requiring high stability and high activity of proteins.