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肺微血管内皮细胞的分离和培养是近年来出现的新技术，文献报道的方法主要有组织块培养法、酶消化法和微载体法等。由于该技术出现的时间不长，不少细节尚待进一步改进和完善。本文主要探讨肺循环灌注对组织块培养法分离、培养大鼠肺微血管内皮细胞（ｒａｔｐｕｌｍｏｎａｒｙ
The isolation and culture of pulmonary microvascular endothelial cells is a new technology emerging in recent years. The methods reported in the literature mainly include tissue culture, enzymatic digestion and microcarrier methods. Due to the appearance of the technology is not long, many details need to be further improved and improved. This article mainly discusses the pulmonary perfusion on tissue culture method isolation, rat pulmonary microvascular endothelial cells (ratpulmonary