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应用免疫组织化学ＳＰ法对４例饲喂黄曲霉毒素Ｂ１的Ｗｉｓｔａｒ大鼠肝组织中ＡＦＢ１－ＤＮＡ加合物的染色及常规ＨＥ染色发现，４例肝细胞核均出现异型性，但未见肝细胞坏死、增生灶及肝细胞癌；免疫组化揭示部分肝细胞核出现棕黄色、不均质状的ＡＦＢ１－ＤＮＡ加合物，阳性细胞数占１０～８５％。结果提示免疫组织化学方法可作为一种ＡＦＢ１的定位方法，ＡＦＢ１在动物致癌过程中ＡＦＢ１－ＤＮＡ加合物可能具有启动作用
Immunohistochemical SP method AFB1-DNA adduct staining and routine HE staining in four Wistar rats aflatoxin B1-fed Wistar rat liver tissue found that all four cases of liver cell nucleus were atypia, but no hepatocytes Necrosis, hyperplasia and hepatocellular carcinoma. Immunohistochemistry revealed AFB1-DNA adduct with brown-yellow and inhomogeneous appearance in some hepatocytes. The number of positive cells was 10-85%. The results suggest that immunohistochemistry can be used as a localization method for AFB1, AFB1-DNA adduct may have a priming effect in animal carcinogenesis