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目的研究过氯酸铵(AP)及碘化钾(KI)联合作用对人甲状腺细胞(nthy-ori3-1)氧化应激及诱导凋亡的影响。方法体外培养nthy-ori3-1细胞,不同浓度AP(0、5、10、20、40mmol/L)及KI(5mmol/L)联合染毒后培养24h,CCK8法检测nthy-ori3-1细胞的增殖活力,流式细胞仪检测细胞凋亡率及细胞内活性氧(ROS)产生情况,同时测定氧化损伤指标丙二醛(MDA)、过氧化氢酶(CAT)活力。结果AP抑制nthy-ori3-1细胞增殖具有明显的剂量依赖性。与对照组比较,各组细胞内ROS生成量随着染毒剂量的增加出现降低趋势,除5mmol/L AP剂量组外,差异均有统计学意义(P<0.05),且AP与KI联合染毒剂量组细胞内ROS生成量比单纯高剂量AP染毒(40mmol/L)细胞内ROS生成量有所降低,差异有统计学意义(P<0.05);较高剂量AP染毒(40mmol/L)甲状腺细胞,MDA生成量比对照组有明显升高,差异有统计学意义(P<0.01);各染毒剂量组细胞内CAT活力比对照组有升高的趋势,但差异无统计学意义(P>0.05)。结论 AP和KI联合作用可致nthy-ori3-1细胞出现氧化应激,且二者之间存在明显的剂量-反应关系,碘在一定程度上可缓解AP对甲状腺细胞的氧化应激反应。
Objective To investigate the effects of combined action of ammonium perchlorate (AP) and potassium iodide (KI) on oxidative stress and apoptosis in human thyrocytes (nthy-ori3-1). Methods NTY-ori3-1 cells were cultured in vitro and incubated with different concentrations of AP (0, 5, 10, 20 and 40 mmol / L) and KI (5 mmol / L) Proliferative activity, apoptotic rate and intracellular reactive oxygen species (ROS) production by flow cytometry. The oxidative damage indexes malondialdehyde (MDA) and catalase (CAT) activity were also measured. Results AP inhibited the proliferation of nthy-ori3-1 cells in a dose-dependent manner. Compared with the control group, the intracellular ROS production decreased with the increase of exposure dose, except for the 5 mmol / L AP dose group, the difference was statistically significant (P <0.05), and AP combined with KI staining The amount of intracellular ROS production in the toxic dose group was lower than that in the high dose AP exposure (40 mmol / L), the difference was statistically significant (P <0.05) ) Thyroid cells, MDA production was significantly higher than the control group, the difference was statistically significant (P <0.01); CAT activity in each dose group than the control group there is an upward trend, but the difference was not statistically significant (P> 0.05). Conclusion The combination of AP and KI can induce oxidative stress in nthy-ori3-1 cells, and there is a significant dose-response relationship between the two. Iodine can alleviate the oxidative stress response of AP to thyroid cells to a certain extent.