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应用McAb的APAAP免疫组化技术对肾综合征出血热(HFRS)患者外周血淋巴细胞的Tac(CD25),TLiSA1,TfR(CD71)和Ia四种活化抗原表达进行了研究,结果表明,HFRS患者急性期外周血淋巴细胞的各种活化抗原表达明显高于恢复期,急性期分别为:36.4±9.3％,36.5±8.4％,43.1±14.3％,42.3±8.0％;恢复期分别为20.0±6.6％,18.9±4.6％22.4±8.5％,23.6±3.9％。对Percoll纯化后的异型淋巴细胞和小细胞各种活化抗原表达进行检测的结果提示,异型淋巴细胞中各种活化抗原的表达显著高于小细胞,异型淋巴细胞分别为:48.1±13.0％,52.1±12.9％,55.7±8.7％,52.7±6.7％;小细胞分别为:12.9±2.3％,13.9±2.7％,15.1±2.9％,23.5±2.6％。此结果对于了解患者机体免疫状态及异型淋巴细胞来源和功能有一定的意义。
The expression of Tac (CD25), TLiSA1, TfR (CD71) and Ia in peripheral blood lymphocytes of patients with HFRS was detected by McAb APAAP immunohistochemistry. The results showed that HFRS patients The expression of various activated antigens in acute peripheral blood lymphocytes were significantly higher than those in recovery phase. The acute phase were 36.4 ± 9.3%, 36.5 ± 8.4%, 43.1 ± 14.3% and 42.3 ± 8.0%, respectively. The recovery period was 20.0 ± 6.6 %, 18.9 ± 4.6% 22.4 ± 8.5%, 23.6 ± 3.9%. The results of Percoll-purified allogeneic lymphocytes and the expression of various activated antigens in small cells indicated that the expression of various activated antigens in allogeneic lymphocytes was significantly higher than that of small cells, and the atypical lymphocytes were 48.1 ± 13.0% and 52.1 ± 12.9%, 55.7 ± 8.7% and 52.7 ± 6.7% respectively; the small cells were 12.9 ± 2.3%, 13.9 ± 2.7%, 15.1 ± 2.9% and 23.5 ± 2.6% respectively. The results for understanding the patient’s immune status and atypical lymphocyte origin and function of some significance.