老年性记忆减退大鼠内侧隔核-斜角带NGFR和NOS神经元各亚群的改变

• 作 者: 王怀星 姚志彬 顾耀铭 陈以慈
• 机 构: 中山医科大学解剖教研室
• 出 处: 暨南大学学报(自然科学与医学版)
• 发表日期: 1998年06期
• 关键词: NOS神经元 斜角带 NGFR 内侧隔核 大鼠 老年性记忆减退 一氧化氮合酶 水迷宫行为学 逃避潜伏期 亚群

本研究采用健康雄性ＳＤ大鼠，青年组（３月龄）２０只，老年组（２６月龄）４０只，通过Ｍｏｒｉｓ水迷宫行为学测试，将老年大鼠分为老年记忆减退组（简称老年减退组）和老年记忆正常组（简称老年正常组）。用免疫细胞化学、组织化学双标技术定量分析内侧隔核－斜角带（ｓｅｐｔｕｍｍｅｄｉａｌｉｓ－ｄｉａｇｏｎａｌｂａｎｄｏｆＢｒｏｃａ，ＳＭ－ＤＢ）中神经生长因子受体（ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒ，ＮＧＦＲ）、一氧化氮合酶（ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ＮＯＳ）阳性神经元各亚群。老年减退组大鼠和老年正常组大鼠相比较，ＳＭ、斜角带垂直支（ｖｅｒｔｉｃａｌＤＢ，ｖＤＢ）、斜角带水平支（ｈｏｒｉｚｏｎａｌＤＢ，ｈＤＢ）中ＮＧＦＲ－ＮＯＳ双标神经元数量明显下降（Ｐ＜００１），其下降幅度为２８８４％，２３３３％和２０３５％；ＮＧＦＲ单标神经元在ＳＭ、ｖＤＢ和ｈＤＢ中也呈明显下降，其下降值为４０４３％，４３２４％，３３０６％；ＮＯＳ单标神经元的数量较老年正常组有显著下降（Ｐ＜００５），下降幅度分别为１７２４％，２０００％和１８６０％。相关分析表明，受试大鼠逃避潜伏期与双标神经元、ＮＧＦ?

In this study, healthy male Sprague-Dawley rats were used in this study. Twenty young adults (aged 3 months) and 40 senile adults (aged 26 months) were divided into three groups according to Moris water maze behavioral test: Diminished group) and normal memory group (referred to as elderly normal group). Immunocytochemistry and histochemical double labeling were used to quantitatively analyze the expressions of nerve growth factor receptor (NGFR), nitric oxide synthase (NOS) in septum medialis-diagonal band of Broca (SM-DB) Subpopulations of positive neurons. The numbers of NGFR-NOS double-labeled neurons in vertical axis (verticalDB, vDB) and horizonalDB (hDB) in the aged group were significantly lower than those in the aged group <001), which decreased by 2884%, 2333% and 2035%, respectively. NGFR single-labeled neurons also showed a significant decrease in SM, vDB and hDB, with a decrease of 4043% , 4324% and 3306% respectively. The number of NOS single-labeled neurons decreased significantly (P <005) compared with the normal control group (1724%, 2000% and 1860%, respectively) %. Correlation analysis showed that the escape latency of test rats was similar to that of double labeled neurons, NGF?