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从‘秦美’猕猴桃（ＡｃｔｉｎｉｄｉａｄｅｌｉｃｉｏｓａＣ．Ｆ．ＬｉａｎｇｅｔＡ．Ｒ．Ｆｅｒｇｕｓｏｎ．ｃｖ．Ｑｉｎｍｅｉ）呼吸高峰跃变初期的果实中提取总ＲＮＡ，然后纯化得ｍＲＮＡ，用Ｏｌｉｇｏ（ｄＴ）作为引物反转录合成ｃＤＮＡ第一链，以此为模板，用人工合成的寡聚核苷酸作为引物进行扩增，得到大小约０．９５Ｋｂ基因片段，并将其克隆到ｐＧＥＭ－５ｚｆ（＋）载体上。经限制性内切酶图谱分析，组建亚克隆并进行了全序列测定，在其开放读码框架内，由９５７个ｂｐ编码３１９个氨基酸组成，该ｃＤＮＡ与海沃德ＡＣＣ氧化酶ｃＤＮＡ的核苷酸和氨基酸残基同源率分别达９５．０１％和９５．６１％，证明己克隆到完整的秦美猕猴桃ＡＣＣ氧化酶基因编码区。
Total RNA was extracted from the fruits of the early stage of respiratory peak jump of ActinidiadeliciosaC.F.LiangetA.R.Ferguson.cv.Qin-mei, and then purified to obtain mRNA, reverse-transcribed with Oligo (dT) as a primer The first strand of cDNA was synthesized and amplified with a synthetic oligonucleotide as a primer to obtain a fragment of about 0.95 kb in size and cloned into the pGEM-5zf (+) vector. After restriction endonuclease mapping analysis, subclones were constructed and sequenced. The open reading frame consisted of 957 bp encoding 319 amino acids. The cDNA was homologous to the nucleoside of Hayward ACC oxidase cDNA The homology of acid and amino acid residues were 95.01% and 95.61%, respectively, which proved that they had been cloned into the complete coding region of ACC oxidase gene of Actinidia americana.