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目的：探讨白血病耐药细胞的发生机制与细胞内钙离子浓度的关系。方法：分别用Ｆｕｒａ２／Ａｍ方法测定细胞内游离钙浓度及ｄｏｔ杂交法测定细胞ｍｄｒ１ｍＲＮＡ表达。结果：发现ＣＡｓ（Ｖｅｒ或Ｄｉｌ）有降低肿瘤细胞内Ｃａ２＋浓度和降低ｍｄｒ１ｍＲＮＡ表达的作用，ＣａＭＡｓ虽无降低Ｃａ２＋浓度的作用，但可使ｍｄｒ１ｍＲＮＡ表达降低，从而降低了Ｋ５６２／ＶＣＲ细胞的耐药性。结论：细胞内Ｃａ２＋浓度的变化可能与ＭＤＲ产生有一定关系；ＣＡｓ导致的Ｃａ２＋浓度的变化可能也是其逆转肿瘤细胞ＭＤＲ的机理之一，ＣＡｓ及ＣａＭＡｓ尚可影响耐药性肿瘤细胞的ｍｄｒ１ｍＲＮＡ表达
Objective: To investigate the relationship between the mechanism of leukemia drug-resistant cells and intracellular calcium concentration. METHODS: The intracellular free calcium concentration was determined by Fura-2/Am method and the dot-migration method was used to determine the mdr1 mRNA expression. RESULTS: It was found that CAs (Ver or Dil) can reduce the intracellular Ca2+ concentration and decrease the expression of mdr1 mRNA. Although CaMAs have no effect on reducing Ca2+ concentration, they can reduce the expression of mdr1 mRNA and reduce the drug resistance of K562/VCR cells. . Conclusion: The change of intracellular Ca2+ concentration may be related to the production of MDR. The change of Ca2+ concentration induced by CAs may be one of the mechanisms of reversing the MDR of tumor cells. CAs and CaMAs can still affect the expression of mdr1 mRNA in drug-resistant tumor cells.