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本文报道了中国人的淋巴细胞氚标地塞米松特异结合的Kd值和Ro。Scatchart作图法分析显示Ro=6.23±2.38moles/10~6细胞,Kd=2.34±1.46nM,证明了甾体结合的特异性。研究了36名正常中国人(男18名,女18名)淋巴细胞GCR分別为3206±1515位点/细胞,36.41±1901位点/细胞(一点分析法),男女之间差别不显著。对11名手球队员(男6名,女6名,受过训练组T)和11名复旦大学学生(男5名,女6名没受过训练者NT)进行了运动实验,结果显示:力竭运动后无论是T组和NT组淋巴细胞GCR数均比运动前减少(T组从3351减少到1431位点/细胞;NT组3912减少到1588,P<0.05)。虽然,T组运动前后的差值比NF组小(差数561位点/细胞),但统计学检验相差不显著。这些结果提示:运动训练对淋巴细胞GCR的变化作用是不显著的。GCR,乳酸和GC的相关检验结果显示:GCR和乳酸存在着相关(r=-0.466 P<0.05),这些提示,在运动中乳酸可能是调节GCR的一个重要因素。
This article reports the Kd value and Ro for the tritium-labeled dexamethasone-specific binding of Chinese lymphocytes. Scatchart mapping showed that Ro = 6.23 ± 2.38 moles / 10 ~ 6 cells, Kd = 2.34 ± 1.46 nM, demonstrating steroid-binding specificity. The GCRs of 36 normal Chinese (18 male and 18 female) lymphocytes were 3206 ± 1515 cells / cell and 36.41 ± 1901 cells / cell (one-point analysis) respectively. There was no significant difference between male and female. Eleven handball players (6 males, 6 females, trained group T) and 11 Fudan University students (5 males and 6 females untreated NT) were tested for exercise. The results showed that exhaustive exercise The GCR numbers of lymphocytes in both T and NT groups were decreased after exercise (T group from 3351 to 1431 loci / cell; NT group 3912 to 1588, P <0.05). Although the difference between before and after exercise in T group was smaller than that in NF group (difference of 561 points / cell), the difference between the two groups was insignificant. These results suggest that the effect of exercise training on lymphocyte GCR changes is insignificant. Correlation analysis of GCR, lactate and GC showed that there was a correlation between GCR and lactate (r = -0.466 P <0.05). These results suggest that lactate may play an important role in the regulation of GCR during exercise.