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作者根据百日咳毒素可使中国仓鼠卵巢(CHO)细胞发生形态学改变这一现象,发展了一种简单的测定百日咳毒素及抗毒素滴度的方法,并将其标准化.毒素滴定直接在微量板中进行.每孔加入25μl经2倍稀释的毒素样品,将0.2ml培养基(含10,000个经胰蛋白酶消化的CHO细胞)加至每孔中,混匀后,将板置于含3.5%CO_2、湿度为98%的培养箱中37℃48小时,使全部细胞融合为1个细胞致病单位(CPU)为毒素滴定终点.百口咳毒素50～100pg/ml以上者在微量板上显示出CHO细胞最大数量融合;低于50～100pg/ml,仅部分融合;而未感染者则
Based on the observation that pertussis toxin causes morphological changes in Chinese hamster ovary (CHO) cells, a simple method for the determination of pertussis toxin and antitoxin titers has been developed and standardized, and the toxin titration is performed directly on the microplate .Add 25 μl of 2-fold diluted toxin sample to each well and add 0.2 ml of medium (containing 10,000 trypsinized CHO cells) to each well. After mixing, place the plate in a solution containing 3.5% CO 2, In a 98% incubator at 37 ° C for 48 hours, all cells were fused to a cell pathogenic unit (CPU) for the end of the toxin titration.50 to 100 pg / ml of 100 keratotoxin showed microcyst on CHO cells The maximum number of fusion; less than 50 ~ 100pg / ml, only partial fusion;