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本实验以二甲氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌的动物模型为材料，观察了在诱癌过程中和肝癌形成后大白鼠肝细胞质膜上几种酶活性的变化。用不连续蔗糖密度梯度离心法制备肝细胞质膜，用分光光度法对酶活性进行定量测定。实验结果表明，在诱癌过程中，肝细胞质膜上５＇－ＡＭＰ酶活性下降，γ－ＧＴ_(ａｓｅ)酶活性显著升高。γ－ＧＴ_(ａｓｅ)酶活性升高幅度与病理变化正相关，并且在诱癌早期就能表现出来。
In this experiment, animal models of liver cancer induced by dimethylaminoazobenzene (DAB) were used as materials to observe the changes of several enzyme activities in the plasma membrane of hepatocytes in the process of carcinogenesis and liver cancer formation. The plasma membrane of hepatocytes was prepared by discontinuous sucrose density gradient centrifugation, and the enzyme activity was measured by spectrophotometry. The experimental results showed that in the process of carcinogenesis, the 5’-AMP enzyme activity on the plasma membrane of hepatocytes decreased, and the activity of γ-GT_ (ase) enzyme increased significantly. The increase in the activity of γ-GT_ (ase) enzyme is positively correlated with pathological changes and can be demonstrated early in cancer induction.