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Ｅｂｓｅｌｅｎ，２－ｐｈｅｎｙｌ（－１，２－ｂｅｎｚｉｓｏｓｅｌｅｎａｚｏｌ－３（２Ｈ）－ｏｎｅ），被谷胱甘肽激活后可与扑热息痛的毒性代谢产物ＮＡＰＱＩ反应，但不能与扑热息痛本身起反应。激活形式的ｅｂｓｅｌｅｎ与ＮＡＰＱＩ反应的阈浓度为２．５μｍｏｌ／Ｌ，ｅｂｓｅｌｅｎ单独与ＮＡＰＱＩ反应的阈浓度为５０μｍｏｌ／Ｌ，且激活形式的ｅｂｓｅｌｅｎ比ｅｂｓｅｌｅｎ本身与ＮＡＰＱＩ反应更快而完全。比较２０μｍｏｌ／Ｌ（ｅｂｓｅｌｅｎ与ＧＳＨ）＋２０μｍｏｌ／ＬＮＡＰＱＩ与２０μｍｏｌ／Ｌ（ｅｂｓｅｌｅｎ与ＧＳＨ）＋２０μｍｏｌ／Ｌ扑热息痛的紫外光措，提示ＮＡＰＱＩ被ｅｂｓｅｌｅｎ还原成其母体化合物扑热息痛，反应的速度受ｅｂｓｅｌｅｎ的浓度，ＮＡＰＱＩ的浓度的影响，反应的最佳ｐＨ范围是７．４至８．２。
Ebselen, 2-phenyl (-1,2-benzisoselenazol-3 (2H) -one), which is activated by glutathione, reacts with the toxic metabolite NAPQI of paracetamol but does not react with paracetamol itself. The threshold concentration of activated forms of ebselen and NAPQI was 2.5 μmol / L, that of ebselen alone and NAPQI was 50 μmol / L, and the activated form of ebselen reacted faster and more completely with NAPQI than ebselen itself. The results showed that NAPQI was reduced to its parent compound paracetamol by ebselen and the reaction speed was affected by the concentration of ebselen and the NAPQI of ebselen and GSH by 20 micromol / L (ebselen and GSH) + 20 micromol / L paracetamol. The optimum pH range for the reaction is 7.4 to 8.2.