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为了探讨重组腺病毒为载体的人ＧＭ ＣＳＦ基因转移的表达及对ＨＢＶ复制的影响 ,我们将携带人ＧＭ ＣＳＦ基因的重组腺病毒感染 2 .2 .15ＨｅｐＧ2细胞 ,观察了ＧＭ ＣＳＦ基因转染前后 2 .2 .15ＨｅｐＧ2细胞上清中ＨＢＶＭ的变化。材料与方法一、材料 主要试剂 :携带人ＧＭ
In order to investigate the expression of recombinant human adenovirus-mediated human GM CSF gene transfer and its effect on HBV replication, we infected 2.215HepG2 cells with recombinant adenovirus carrying human GM CSF gene and observed the expression of GM CSF gene before and after 2 .2 .15 Changes in HBVM in HepG2 cell supernatants. Materials and methods First, the material The main reagent: carrying human GM