论文部分内容阅读
目的:建立胶束电动毛细管电泳(MECC)分离测定紫杉醇的新方法。方法:以40.2 cm×50μm(到检测窗口的有效长度30 cm)的熔融石英毛细管柱为分离柱、V(10 mmol/L三羟甲基氨基甲烷+140 mmol/L十二烷基硫酸钠+70 mmol/L硼酸+40 mmol/L羟丙基-β-环糊精):V(甲醇+异丙醇)=1:1(其中V(甲醇):V(异丙醇)=1:1)为分离缓冲液,于203 nm处检测。结果:方法的精密度为0.08%(n=7),检出限为1.5 mg/L(S/N=3),定量限为4.5 mg/L(S/N=10),1200 mg/L紫杉醇的迁移时间的相对标准偏差(RSD)为0.18%,峰面积的RSD为2.5%。用校正峰面积归一化法测定了纯化后的紫杉醇的主成分含量,并与高效液相色谱(HPLC)的测定结果进行了比较。
Objective: To establish a new method for the separation and determination of paclitaxel by micellar electrokinetic capillary electrophoresis (MECC). Methods: A fused silica capillary column (40.2 cm × 50 μm (effective length 30 cm) was used as the separation column. V (10 mmol / L trimethylolaminomethane + 140 mmol / L sodium lauryl sulfate + 70 mmol / L boric acid + 40 mmol / L hydroxypropyl-β-cyclodextrin): V (methanol + isopropanol) = 1: 1 ) As the separation buffer and detected at 203 nm. Results: The precision of the method was 0.08% (n = 7) and the limit of detection was 1.5 mg / L (S / N = 3) The relative standard deviation (RSD) for paclitaxel migration time was 0.18% and the peak area RSD was 2.5%. The principal component content of purified paclitaxel was determined by the normalized calibration peak area method and compared with the results of high performance liquid chromatography (HPLC).