论文部分内容阅读
目的 :建立一种简单可靠的骨髓基质细胞 (BMSc)向成骨细胞转化的体外培养方法 ,探讨骨髓基质细胞向成骨细胞转化的机理。 方法:将兔 BMSc悬液进行体外培养 ,进行形态学观察和组织学检测。 结果:传代培养细胞的碱性磷酸酶 (AL P)阳性率达 80 %以上 ,表现为成骨细胞形态并形成钙结节。结论:地塞米松、β-甘油磷酸钠和维生素 C可促进 BMSc分化为成骨细胞 ,BMSc的分化和增殖是两个对立的状态。
Objective: To establish a simple and reliable method of culturing bone marrow stromal cells (BMSc) into osteoblasts in vitro and to explore the mechanism of bone marrow stromal cells transforming into osteoblasts. Methods: Rabbit BMSc suspension was cultured in vitro, morphological observation and histological examination. Results: The positive rate of alkaline phosphatase (ALP) in cultured cells was more than 80%, which showed osteoblast morphology and formation of calcium nodules. Conclusion: Dexamethasone, β-glycerophosphate and vitamin C can promote the differentiation of BMSc into osteoblasts. The differentiation and proliferation of BMSc are two opposite states.