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目的:探讨中药香加皮提取物三萜类化合物(triterpenes compound of cortex periplocae,TCCP)对甲基苄基亚硝胺(N-nitrosomethylbenzylamine,NMBA)诱导的大鼠食管癌组织中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达的影响。方法:雄性F344大鼠120只,随机分为NMBA模型组、TCCP干预组、大豆油对照组和正常对照组。模型组大鼠皮下注射NMBA,TCCP干预组大鼠同时给予皮下注射NMBA及肌注TCCP,大豆油对照组大鼠肌注大豆油,正常对照组大鼠常规饲养。分别在给药后第9、15和25周,H-E染色检测大鼠食管上皮组织病理变化,免疫组织化学SP法检测大鼠食管组织中PCNA的表达。结果:正常对照组及大豆油对照组大鼠食管未发现异常变化,NMBA模型组大鼠9周时,食管癌前病变发生率为20.0%,15周时为46.7%,25周时达93.3%。与NMBA模型组相比,第9、15周时TCCP干预组癌前病变大鼠的比例明显降低(0、0 vs 20.0%、46.7%,P<0.05)。NMBA模型组第9、15和25周时,大鼠食管上皮组织PCNA表达水平显著高于正常对照组(213.17±29.74 vs 167.96±20.16,268.35±39.56 vs 170.76±14.79,327.24±28.19 vs 172.49±17.49;P<0.05);与NMBA模型组相比,TCCP干预组大鼠食管上皮组织中PCNA表达水平显著降低(P<0.05)。结论:TCCP可抑制NMBA诱导的大鼠食管癌前病变,该作用可能与其抑制PCNA的表达有关。
OBJECTIVE: To investigate the effect of triterpenes compound of cortex periplocae (TCCP) on proliferating cell nuclear antigen (PCNA) in rat esophageal cancer induced by N-nitrosomethylbenzylamine (NMBA) cell nuclear antigen, PCNA expression. Methods: 120 male F344 rats were randomly divided into NMBA model group, TCCP intervention group, soybean oil control group and normal control group. Rats in model group were subcutaneously injected with NMBA. Rats in TCCP intervention group were given subcutaneous injection of NMBA and intramuscular injection of TCCP. Soybean oil control rats were intramuscularly injected with soybean oil and rats in normal control group were routinely housed. At the 9th, 15th and 25th week after administration, the pathological changes of esophageal epithelium were detected by H-E staining and the expression of PCNA in rat esophageal tissue was detected by immunohistochemical SP method. RESULTS: No abnormal changes of esophagus were observed in normal control group and control group. The incidence of esophageal precancerous lesions was 20.0% at 9 weeks in NMBA model group, 46.7% at 15 weeks and 93.3% at 25 weeks. Compared with the NMBA model group, the proportion of precancerous lesions in the TCCP intervention group was significantly decreased at the 9th and 15th weeks (0,0 vs 20.0%, 46.7%, P <0.05). At the 9th, 15th and 25th week in NMBA model group, the expression level of PCNA in rat esophageal epithelium was significantly higher than that in the normal control group (213.17 ± 29.74 vs 167.96 ± 20.16, 268.35 ± 39.56 vs 170.76 ± 14.79, 327.24 ± 28.19 vs 172.49 ± 17.49 ; P <0.05). Compared with NMBA model group, the expression level of PCNA in esophageal epithelium significantly decreased in TCCP intervention group (P <0.05). Conclusion: TCCP can inhibit NMBA-induced esophageal precancerous lesions in rats, which may be related to the inhibition of PCNA expression.