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目的探讨丹参提取物764-3和超氧化物歧化酶(SOD)对高氧损伤新生大鼠脑细胞的保护作用。方法将SD新生大鼠持续暴露于氧浓度≥90%的高氧环境中建立高氧脑损伤模型,然后分别予764-3、SOD进行预防(1~7d)和治疗(8~14d)干预后,计算各组大鼠存活率、体质量和脑湿/干重比值;采用TUNEL技术检测大鼠脑细胞凋亡情况并计算凋亡指数(AI)。结果与空气对照组比较,高氧对照组的AI显著上升(P<0.01);干预组的AI均显著低于高氧对照组(P<0.01),预防组的AI显著高于治疗组(P<0.01)。结论 764-3和SOD对于高氧脑损伤均有一定的保护作用,且治疗作用优于预防作用。
Objective To investigate the protective effect of Salvia miltiorrhiza extract 764-3 and superoxide dismutase (SOD) on brain cells of neonatal rats with hyperoxia injury. Methods The neonatal SD rats were exposed to hyperoxia (≥90% oxygen) environment to establish the model of hyperoxic brain injury. Then 764-3 SD rats were treated with prophylactic (1-7 d) and 8-10 d The survival rate, body weight and wet / dry weight ratio of the rats in each group were calculated. The apoptosis of brain cells was detected by TUNEL technique and the apoptosis index (AI) was calculated. Results Compared with air control group, AI in hyperoxia group increased significantly (P <0.01), AI in intervention group was significantly lower than that in hyperoxia group (P <0.01), AI in prevention group was significantly higher than that in treatment group <0.01). Conclusion 764-3 and SOD have some protective effects on hyperoxic brain injury, and the therapeutic effect is better than the preventive effect.