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目的:研究鹰爪花Artabotrys hexapetalus茎叶的显微结构及其内生真菌在组织内的分布特点及种类鉴定。方法:采用石蜡永久制片法对鹰爪花的显微结构进行研究,细胞化学法确定内生真菌的分布,平板分离法对内生真菌分离培养,通过rDNA中内转录间隔区(ITS)序列进行分类鉴定。结果:鹰爪花茎次生构造由周皮、皮层和维管柱组成,韧皮部纤维成层分布;叶为异面叶,主脉维管束为外韧型,具纤维组成的维管束鞘;茎和叶多个组织有内生菌分布。共分离鉴定18株内生真菌,其中拟茎点霉Phomopsis spp.是鹰爪花内生真菌优势菌群,占分离总数的61.1%,其次为胶孢刺盘孢Colletotrichumgloeosporioides。结论:鹰爪花茎和叶显微特征明显,其内生真菌的分布无明显组织特异性,拟茎点霉为鹰爪花内生真菌的优势种群。
Objective: To study the microstructure of stem and leaf of Artabotrys hexapetalus and its distribution characteristics and species identification of endophytic fungi in tissues. Methods: The microstructure of Talus flower was studied by permanent paraffin method. The distribution of endophytic fungi was determined by cytochemical method. Endophytic fungi were isolated and cultured by plate separation method. The internal transcribed spacer (ITS) sequence For classification identification. Results: The secondary structure of the cauliflower stems was composed of periderm, cortex and vascular column. The phloem fibers were distributed in layers. The leaves were heterogenous leaves. The vascular bundles of the main veins were external tough type with vascular bundle sheath composed of fibers. Leaf tissue distribution of endophyte. A total of 18 strains of endophytic fungi were isolated and identified. Phomopsis spp. Was the dominant group of endophytic fungi, accounting for 61.1% of the total isolates, followed by Colletotrichum gloeosporioides. CONCLUSION: The morphological characteristics of stem and leaf of H. palmi have obvious microstructure. The distribution of endophytic fungi has no obvious tissue specificity. Phomopsis phomori is the dominant species of endophytic fungus.