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目的研究过量氟在大鼠体内的蓄积以及钙的拮抗作用。方法将80只初断乳清洁级SD大鼠随机分为8组,分别为对照(蒸馏水)组、单独加钙(100 mg/kg碳酸钙)组和低剂量(2.1 mg/kg Na F)、中剂量(8.5 mg/kg Na F)、高剂量(34 mg/kg Na F)单独染氟组及低剂量(2.1 mg/kg Na F+25 mg/kg碳酸钙)、中剂量(8.5 mg/kg Na F+50 mg/kg碳酸钙)、高剂量(34 mg/kg Na F+100 mg/kg碳酸钙)钙氟联合染毒组,每组10只,雌雄各半。采用灌胃方式进行染毒,每天1次,连续染毒90 d。每15 d测定24 h尿氟浓度,染毒结束后测定骨氟含量。结果与对照组比较,低、中、高剂量单独染氟组和中、高剂量钙氟联合染毒组大鼠骨氟含量升高,低、中、高剂量单独染氟组和低、中、高剂量钙氟联合染毒组大鼠尿氟浓度升高,差异有统计学意义(P<0.05)。随着氟暴露剂量的升高,大鼠的骨氟含量和尿氟浓度均呈上升趋势。与相同剂量单独染氟组比较,钙氟联合染毒组大鼠骨氟含量有所降低,差异多有统计学意义(P<0.05);而不同性别大鼠在不同钙氟联合染毒组、不同时间点下,钙对尿氟浓度的拮抗作用程度不尽相同。结论适量的钙可拮抗氟在大鼠体内的蓄积作用。
Objective To study the accumulation of excess fluoride in rats and the antagonism of calcium. Methods 80 SD rats were randomly divided into 8 groups: control group (distilled water), calcium alone (100 mg / kg) and low dose (2.1 mg / kg NaF) (8.5 mg / kg Na F), high dose (34 mg / kg Na F) alone and low dose (2.1 mg / kg Na F +25 mg / kg calcium carbonate) kg Na F + 50 mg / kg calcium carbonate) and high dose (34 mg / kg NaF + 100 mg / kg calcium carbonate) calcium and fluorine combination group. Gavage by way of exposure, 1 times a day, continuous exposure 90 d. Determination of urinary fluoride 24 h every 15 d, determination of bone fluoride content after the end of the exposure. Results Compared with the control group, low, medium and high doses of fluoride alone group and medium and high doses of calcium and fluorine combined with fluoride group rats increased fluoride content, low, medium and high doses of fluoride alone group and low, The urinary fluoride concentration increased in high-dose calcium-fluoride combined group rats, the difference was statistically significant (P <0.05). With the increase of fluoride exposure, the bone fluoride content and urinary fluoride concentration in rats showed an upward trend. Compared with the same dose of fluoride group alone, the content of fluoride in bone of rats in the combination of calcium and fluorine decreased slightly, the difference was statistically significant (P <0.05); while different gender rats in different calcium and fluorine combined exposure group, At different time points, the antagonistic effects of calcium on urinary fluoride concentrations varied. Conclusion Appropriate amount of calcium can antagonize the accumulation of fluoride in rats.