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目的:探讨次乌头碱(HA)与甘草苷、甘草次酸配伍对心肌细胞的减毒作用。方法:以新生SD乳鼠心肌细胞为研究对象,观察甘草苷、甘草次酸对HA引起心肌细胞的形态变化和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性的影响。结果:与120μmol/L HA组比较,120μmol/L甘草次酸、480μmol/L甘草苷与120μmol/L HA配伍可使SOD、GSH-PX的活性由(56.38±0.41)U/mL和(81.89±3.67)U/mgprot分别增加到(65.39±0.25)U/mL、(101.69±7.17)U/mgprot和(62.69±0.54)U/mL、(100.85±7.32)U/mgprot(P<0.05),120μmol/L的甘草次酸和120μmol/L HA配伍作用更显著(P<0.01)。结论:480μmol/L甘草苷、120μmol/L甘草次酸与120μmol/L HA配伍可以降低HA对心肌细胞的毒性作用,增强其活力,这与甘草苷、甘草次酸减少了HA对心肌细胞的氧化损伤有关。
Objective: To investigate the attenuated effect of hypaconitine (HA) combined with glycyrrhizin and glycyrrhetinic acid on cardiomyocytes. Methods: Newborn SD neonatal rat cardiomyocytes were used to study the effects of glycyrrhizin and glycyrrhetinic acid on the morphological changes of cardiomyocytes induced by HA and the effects of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) ) Activity. Results: Compared with 120μmol / L HA group, the activities of SOD and GSH-PX in the groups of 120μmol / L glycyrrhetinic acid, 480μmol / L glycyrrhizin and 120μmol / L HA increased from (56.38 ± 0.41) U / mL and (81.89 ± 3.67) U / mgprot increased to (65.39 ± 0.25) U / mL, (101.69 ± 7.17) U / mgprot and (62.69 ± 0.54) U / mL respectively and (100.85 ± 7.32) U / / L glycyrrhetinic acid and 120μmol / L HA compatibility effect more significant (P <0.01). CONCLUSION: The combination of 480μmol / L glycyrrhizin, 120μmol / L glycyrrhetinic acid and 120μmol / L HA can reduce the toxic effect of HA on cardiomyocytes and increase the activity of glycyrrhizin. Glycyrrhizin and glycyrrhetinic acid can reduce the oxidation of myocardial cells Damage related.