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以阻止熟蚕吐丝的方法建立家蚕高蛋白血症模型,研究模型家蚕丝腺退化过程中细胞程序性死亡的特点,探索家蚕高蛋白血症模型的发病机制。形态解剖学观察显示,模型家蚕的丝腺退化过程延长了2倍时间;组织切片HE染色显示,模型家蚕的中部丝腺和后部丝腺都出现了退化启动推迟的现象。对家蚕组织解体退化信号通路相关基因表达水平的检测显示,模型家蚕丝腺细胞中自噬信号基因Atg6和Atg8的转录水平显著低于对照组家蚕,而凋亡信号基因Dronc的转录水平则显著高于对照组家蚕。免疫组化染色显示,模型家蚕丝腺细胞中凋亡启动蛋白Caspase-3在丝腺退化过程中持续表达。研究结果表明高蛋白血症模型家蚕丝腺细胞的凋亡活动增强,而丝腺退化延缓与自噬作用启动延迟有关。
To prevent silkworm silkworm silkworm silkworm silkworm hyperlipoproteinemia model established to study silkworm silkworm degenerative process programmed cell death characteristics of silkworm hyperlipoproteinemia model to explore the pathogenesis. Morphological and anatomical observations showed that silkworm degenerative process in silkworms was prolonged by 2 times. The histological sections of silkworm showed delayed degenerative initiation in both silk gland and posterior silk gland. The results showed that the transcription level of Atg6 and Atg8 in silk gland cells was significantly lower than that in control silkworm, while the transcription level of Dronc was significantly higher than that in silkworm Control group, silkworm. Immunohistochemical staining showed that the expression of Caspase-3, an inhibitor of apoptosis protein in silk gland cells, was continuously expressed in silk gland degradation. The results showed that the apoptosis of silk gland cells in the model of hyperproteinemia was enhanced, while the delayed degradation of silk gland was related to the delayed initiation of autophagy.