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目的了解乌鲁木齐地区婴幼儿轮状病毒腹泻流行情况。方法收集新疆自治区人民医院2007年1～12月住院和门诊0～60月龄婴幼儿腹泻标本376份,ELISA筛查轮状病毒(RV),用RT-PCR对RV毒株进行基因分型。结果共检测376份标本,轮状病毒阳性181份,阳性率48.14%。感染者主要为出生24个月以内的婴幼儿(占98.34%)。9～10月为RV腹泻流行高峰。对161份阳性标本进行G/P基因分型,G1型是主要优势株,占31.68%,其次为G9型(21.12%)、G3型(15.53%)和G2型(14.91%),混合G型感染占14.29%,4例(2.48%)未能分型;P基因型以P[8]为主,占46.58%,其次为P[6](18.63%)、P混合感染(18.01%)和P[4](14.91%),3例(1.86%)未能分型;常见G/P组合G1P[8](24.84%)。结论轮状病毒是乌鲁木齐地区婴幼儿腹泻的主要病原,2007年G1P[8]是主要流行株,G9型成为本地区的第二大优势株。
Objective To understand the prevalence of infant rotavirus diarrhea in Urumqi. Methods 376 infants and young children with diarrhea aged 0 ~ 60 months from January to December 2007 in People’s Hospital of Xinjiang Uygur Autonomous Region were enrolled in this study. Rotavirus (RV) was screened by ELISA and genotyped by RT-PCR. Results A total of 376 specimens were detected, with 181 rotavirus positive, the positive rate was 48.14%. Infected mainly infants and young children within 24 months (98.34%). 9 to 10 for the peak RV diarrhea epidemic. G / P genotyping was performed on 161 positive samples. Type G1 was the predominant strain, accounting for 31.68%, followed by G9 (21.12%), G3 (15.53%) and G2 (14.91%). P (8) accounted for 46.58%, followed by P [6] (18.63%), P mixed infection (18.01%) and P P [4] (14.91%), 3 cases (1.86%) failed to type; common G / P combination of G1P [8] (24.84%). Conclusions Rotavirus is the major cause of infant diarrhea in Urumqi. In 2007, G1P [8] was the main epidemic strain and G9 was the second dominant strain in this region.