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目的研究四逆散干预创伤后应激障碍大鼠的分子生物学机制。方法将SD大鼠50只按照体重平均分为5组:空白对照组、模型组、阴性对照组、阳性对照组、实验组。除了空白对照组以外,其他4组动物均制备模型。空白对照组不接受治疗,正常饲养。模型组不接受治疗。阴性对照组灌胃等体积0.9%Na Cl。阳性对照组灌胃盐酸帕罗西汀溶液0.42 mg·m L~(-1)。实验组灌胃四逆散水煎液(含生药0.24 g·m L~(-1))。于应激造模前1 h灌胃给药10 m L·kg~(-1)。每天灌服1次,共计7 d。于末次给药5 h后,各组统一采集海马组织。用HPLC法分析大鼠后海马组织匀浆的色谱峰。色谱条件:Agilent HC-C18(4.6 mm×250 mm,5μm);流速:0.8 m L·min~(-1);流动相A为甲醇,B为乙腈,C为0.05%磷酸水,梯度洗脱;检测波长:260 nm;柱温:23℃。结果与空白对照组1号、5号的峰面积(220.40±9.25),(86.27±7.39)m AU×min比较,模型组1号峰面积(82.50±9.60)m AU×min明显降低而5号峰面积(123±9.28)m AU×min明显升高,差异有统计学意义(均P<0.05)。与模型组比较,阴性对照组的1号峰面积(95.50±9.89)m AU×min、5号峰面积(119.30±9.93)m AU×min的色谱峰差异无统计学意义(P>0.05)。与阴性对照组比较,阳性对照组和实验组的1号峰面积(230.42±11.21),(234.80±12.47)m AU×min明显升高而5号峰面积(71.29±1.82),(72.16±2.55)m AU×min明显降低,差异有统计学意义(均P<0.05)。与阳性对照组比较,实验组新出现了8号、9号峰,提示这2个峰可能是四逆散内在化学成分或在机体内的代谢产物。结论四逆散对大鼠海马组织分子表达有明显的正向调节作用。
Objective To investigate the molecular mechanism of Sini Powder in the treatment of post-traumatic stress disorder in rats. Methods Fifty SD rats were equally divided into five groups according to body weight: blank control group, model group, negative control group, positive control group and experimental group. In addition to the blank control group, the other four groups of animals were prepared models. Blank control group did not receive treatment, normal feeding. The model group did not receive treatment. The negative control group was orally gavaged with 0.9% NaCl. The positive control group was given paroxetine hydrochloride solution 0.42 mg · m L -1. The experimental group was given Shengsi Decoction (containing crude drug 0.24 g · m L -1). Administration of 10 m L · kg ~ (-1) at 1 h before modeling. Served 1 day, a total of 7 d. After the last administration for 5 h, the hippocampus tissues were collected uniformly from each group. The chromatographic peaks of hippocampal tissue homogenates were analyzed by HPLC. Chromatographic conditions: Agilent HC-C18 (4.6 mm × 250 mm, 5 μm); flow rate: 0.8 m L · min -1; mobile phase A was methanol; B was acetonitrile; ; Detection wavelength: 260 nm; column temperature: 23 ℃. Results Compared with blank control group, the peak area of No. 1 and No. 5 (220.40 ± 9.25) and (86.27 ± 7.39) m AU × min, the peak area of model No. 1 (82.50 ± 9.60) m AU × min decreased obviously while the number of No.5 Peak area (123 ± 9.28) m AU × min significantly increased, the difference was statistically significant (P <0.05). Compared with the model group, there was no significant difference in peak area (95.50 ± 9.89) m AU × min and peak area (119.30 ± 9.93) m AU × min in the negative control group (P> 0.05). Compared with the negative control group, the peak area of No. 1 (230.42 ± 11.21) and (234.80 ± 12.47) m AU × min in the positive control group and the experimental group were significantly increased while the peak area of No. 5 (71.29 ± 1.82) and (72.16 ± 2.55) ) m AU × min significantly decreased, the difference was statistically significant (P <0.05). Compared with the positive control group, the experimental group appeared on the 8th and 9th peaks, suggesting that these two peaks may be the intrinsic chemical components of Siniger or the metabolites in the body. Conclusion Sini Decoction has significant positive effect on the molecular expression of hippocampus in rats.