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目的研究系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)Nr4a1基因m RNA表达水平。方法通过Ficoll梯度离心法分离出47例SLE患者和30例健康人PBMCs,用TRIzol提取总RNA,再将总RNA逆转录成c DNA,以荧光定量PCR法检测Nr4a1基因m RNA水平,比较SLE患者组和健康对照组的Nr4a1基因m RNA水平差别,分析SLE患者Nr4a1基因m RNA水平与补体C3、补体C4、SLE疾病活动度指数(SLEDAI)的相关关系。结果与健康对照组比较,SLE患者组PBMCs中Nr4a1基因m RNA水平明显降低,差异有统计学意义。结论 SLE患者PBMCs中Nr4a1基因m RNA水平显著低于健康人。
Objective To study the expression of m RNA of Nr4a1 gene in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus erythematosus (SLE). Methods 47 cases of SLE and 30 healthy individuals were isolated by Ficoll gradient centrifugation. The total RNA was extracted with TRIzol, and the total RNA was reverse transcribed into c DNA. The m RNA level of Nr4a1 gene was detected by fluorescence quantitative PCR. The difference of m RNA level between Nr4a1 gene and healthy control group was analyzed. The relationship between Nr4a1 m RNA level and complement C3, complement C4 and SLE disease activity index (SLEDAI) was analyzed. Results Compared with healthy controls, the level of m RNA in Nr4a1 gene of PBMCs in SLE patients was significantly lower than that in healthy controls (P <0.05). Conclusion The level of m RNA in Nr4a1 gene of PBMCs in patients with SLE is significantly lower than that in healthy people.