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目的:探讨重组人碱性成纤维细胞生长因子(rhbFGF)基因及其蛋白对免冠状动脉血管结构的影响。方法:66只实验兔随机分为心肌内注射基因组、心肌内注射蛋白组、心肌内注射生理盐水组及静脉内注射蛋白组。建立兔急性心肌梗死模型,缺血心肌内分别注射真核表达质粒pcDNA_3-bFGF、rhbFGF蛋白、生理盐水及静脉内注射rhbFGF蛋白,观察免冠状动脉血管结构的变化及血管重塑情况。结果:术后12周,心肌内注射基因组、心肌内注射蛋白组、心肌内注射生理盐水组及静脉内注射蛋白组平均管壁厚度分别为:(39.21±10.46)μm、(29.87±12.96)μm、(18.13±11.33)μm、(20.25±9.56)μm;管壁/管腔比值分别为0.35±0.12、0.33±0.14、0.24±0.09、0.25±0.09。结论:缺血心肌内注射rhbFGF基因及其蛋白均能引起免冠状动脉血管重塑,而基因的作用更显著,静脉内注射蛋白没有观察到明显的血管重塑。
Objective: To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) gene and its protein on the structure of vessel-free coronary artery. Methods: Sixty six rabbits were randomly divided into intramyocardial injection group, intramyocardial injection of protein group, intramyocardial injection of saline group and intravenous injection of protein group. Rabbit models of acute myocardial infarction were established. The eukaryotic expression plasmids pcDNA3-bFGF, rhbFGF protein, saline and rhbFGF protein were injected into the ischemic myocardium to observe the changes of vascular structure and vascular remodeling. Results: At 12 weeks after operation, the mean wall thickness of the myocardium, intramyocardial injection group, intramyocardial injection of saline group and intravenous injection group were (39.21 ± 10.46) μm and (29.87 ± 12.96) μm respectively , (18.13 ± 11.33) μm and (20.25 ± 9.56) μm, respectively. The wall / lumen ratios were 0.35 ± 0.12, 0.33 ± 0.14, 0.24 ± 0.09 and 0.25 ± 0.09, respectively. CONCLUSION: In rhGFGF gene and its protein injected into ischemic myocardium, coronary artery remodeling can be induced, while the effect of gene is more significant. No obvious vascular remodeling is observed in intravenous injection of protein.