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①目的 探讨扩增片段长度多态性（Ａｍ ｐ－ＦＬＰ）连锁分析对杜氏进行性肌营养不良（ＤＭＤ）基因诊断中的价值。②方法 运用多重聚合酶链反应（ＰＣＲ）、聚丙烯酰胺凝胶电泳银染法，对１０ 个ＤＭＤ家系成员的抗肌营养不良蛋白基因内９个基因区进行了Ａｍ ｐ－ＦＬＰ连锁分析。③结果 １０ 个ＤＭＤ家系中的９ 个先证者，６ 例为外显子缺失，１ 例为内含子缺失，２ 例为非缺失型。④结论 Ａｍ ｐ－ＦＬＰ连锁分析是对ＤＭＤ进行基因诊断的实用方法。
Objective To investigate the value of Amp-FLP linkage analysis in the diagnosis of Duchenne’s muscular dystrophy (DMD) gene. Methods Using polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE), Am p-FLP linkage analysis was carried out in 9 gene regions of the dystrophin gene in 10 DMD pedigrees. ③ Results Nine probands in 10 DMD pedigrees, including 6 exons deletion, 1 deletion of intron and 2 non-deletion. ④ Conclusion Am p-FLP linkage analysis is a practical method of genetic diagnosis of DMD.