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新近,一种分析型高速逆流色谱仪已问世。本文首次报道分析型高速逆流色谱(HSCCC)与光电系列检测器(HSCCG-UV联用。由于流动相的作用使固定相液滴流入检测器,使噪音增加。如果在柱后反应器(阻抗器)中存在一种能与流动相和固定相都混溶的助溶剂,那就能使噪音降低到可接受的程度。用HSCCC-UV分析香豆素和一个黄酮的混合物,在不到20min的操作时间内,
Recently, an analytical high-speed countercurrent chromatography has come out. This paper reports for the first time the combination of high speed countercurrent chromatography (HSCCC) with a photoelectric detector (HSCCG-UV), which increases the noise due to the flow of stationary phase droplets into the detector.If the post-column reactor ), A cosolvent that is miscible with the mobile phase and the stationary phase can reduce the noise to an acceptable level.Using HSCCC-UV to analyze the mixture of coumarin and one flavonoid in less than 20 min During operation time,