不孕症42例的TORCH、CBV联合检测

• 作 者: 王喜英 卢清兰 尹慧卿 陈会枝 张莲琴
• 机 构: 河南省卫生厅临床检验中心,淮阳县人民医院,河南医科大学第一附属医院
• 出 处: 中国冶金工业医学杂志
• 发表日期: 1999年03期
• 关键词: TORCH CBV 巨细胞病毒 孕妇组 弓形体 间接酶联免疫法 萨奇 原发不孕 继发不孕 混合感染

目的：探讨ＴＯＲＣＨ（弓形体、风疹病毒和巨细胞病毒的合称）、ＣＢＶ（柯萨奇Ｂ组病毒）感染与不孕症之间的关系。方法：采用抗体捕获酶联免疫和间接酶联免疫法技术，检测４２例不孕症患者血清中ＴＯＲＣＨ、ＣＢＶ特异性抗体ＩｇＭ、ｌｇＧ，并与９２例早孕妇女、２６例健康经产妇、２８例晚孕妇女相对照。结果：（１）不孕症患者ＴＯＲＣＨ总感染率（２３８％）与孕妇组总感染率（５４％），经统计学处理，差别非常显著（Ｐ＜００２）。其中，风疹病毒感染率不孕症组（９５％）与孕妇组感染率（１０８％）比较，存在显著性差异（Ｐ＜００５）；不孕症组弓形体和巨细胞病毒感染率分别为９５％、４７６％，与孕妇组（３６％、１０８％）比较，差别无显著性（Ｐ＞００５）。（２）不孕症组ＣＢＶ－ＩｇＭ感染率（６４３％）与健康经产妇感染率（１５％），经统计学处理，差别非常显著（Ｐ＜０００１）；与孕妇组（３７５％）比较，差别有显著性（Ｐ＜００５）。（３）不孕症组存在两种病原体混合感染情况。结论：提示ＴＯＲＣＨ、ＣＢＶ感染是不孕症的重要病因之一。

Objective: To investigate the relationship between TORCH (Toxoplasma gondii, rubella and cytomegalovirus) and CBV (coxsackie virus B) infertility. Methods: TORCH and CBV specific IgM and IgG were detected in 42 infertile patients by ELISA and indirect enzyme-linked immunosorbent assay (ELISA). The results were compared with 92 pregnant women, 26 healthy women and 28 healthy women Pregnant women relative contrast. Results: (1) The total infection rate of TORCH (238%) in infertility patients and the total infection rate in pregnant women group (54%) were statistically significant (P <002). Among them, the infection rate of rubella virus in infertility group (9.5%) was significantly different from that in the pregnant group (1.08%) (P <005) The rates of virus infection were 95% and 476%, respectively. There was no significant difference (P> 005) between the rate of infection and the pregnant women (3.6%, 1.08%). (2) The infection rate of CBV-IgM in infertility group (643%) and maternal infection rate (15%) were statistically significant (P0.01)  5%), the difference was significant (P <0  05). (3) infertility group there is a mixed infection of two pathogens. Conclusion: It is suggested that TORCH and CBV infection are one of the important causes of infertility.