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用逆转录病毒载体介导人ＩＬ－２基因导入人乳腺癌细胞系ＭＣＦ－７并获得表达。原位杂交结果证实，转染的ＭＣＦ－７细胞中有ＩＬ－２ｍＲＮＡ的表达，用ＩＬ－２依赖细胞系ＣＴＬＬ－２以ＭＴＴ比色法测得ＭＣＦ－７细胞培养上清液中有ＩＬ－２活性，其活性平均为１０ＩＵ／１０￣６细胞。转导后的ＭＣＦ－７细胞对ＬＡＫ细胞的杀伤敏感性降低。
Human IL-2 gene was introduced into human breast cancer cell line MCF-7 by retroviral vector and expressed. The results of in situ hybridization confirmed that IL-2 mRNA was expressed in transfected MCF-7 cells. IL-2 mRNA was detected by MTT colorimetric assay in IL-2-dependent cell line CTLL-2. 2 activity, the average activity of 10IU / 10 ~ 6 cells. The sensitivity of transduced MCF-7 cells to LAK cells was reduced.