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目的为了探讨血小板1bα基因多态性与中国人群不稳定性心绞痛的关系。方法随机收集90例不稳定性心绞痛患者,并按年龄、性别相匹配收集90例健康对照人群,观察不稳定性心绞痛与血小板糖蛋白1bα145Thr/Met变异的关系。采用聚合酶链反应-限制性片段多态性(PCR-RFLP)方法进行血小板糖蛋白1bα145Thr/Met变异的分析。结果不稳定性心绞痛组血小板GP1bα基因145M/M纯合子为2例,145T/M杂合子为16例,突变率20.00%,对照组纯合子为0例,杂合子为11例,突变率12.22%,结果显示两组血小板糖蛋白1bα145Thr/Met差别有显著意义(P=0.004)。OR=2.12,95%CI:1.13～3.18。排除高血压、糖尿病、高血脂、吸烟史、冠心病家族史等因素,受试者UA的发生率与血小板膜GPⅠbα基因145Thr/Met变异仍然有相关性(OR=1.001,95%CI:0.7～1.8)。结论血小板糖蛋白1bα145Thr/Met变异与中国人群不稳定性心绞痛的发生相关。
Objective To investigate the relationship between platelet 1bα gene polymorphism and unstable angina in Chinese population. Methods Ninety patients with unstable angina pectoris were collected at random and 90 healthy controls were collected by age and sex to observe the relationship between unstable angina pectoris and platelet glycoprotein 1bα14Thr / Met mutation. Analysis of platelet glycoprotein 1bα145Thr / Met variation using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results In the group of unstable angina pectoris, 145 M / M homozygotes for platelet GP1bα gene were found in 2 cases, 145 T / M heterozygotes were in 16 cases, the mutation rate was 20.00%, in the control group, 0 cases were homozygous and 11 cases were heterozygous. The mutation rate was 12.22% The results showed that there was significant difference between the two groups in the platelet glycoprotein 1bα145Thr / Met (P = 0.004). OR = 2.12, 95% CI: 1.13 ~ 3.18. Excluding the factors of hypertension, diabetes, hyperlipemia, smoking history, family history of coronary heart disease and other factors, the incidence of UA was still correlated with the 145Thr / Met mutation of platelet membrane GPⅠbα gene (OR = 1.001, 95% CI: 1.8). Conclusion The platelet glycoprotein 1bα145Thr / Met mutation is associated with the occurrence of unstable angina in Chinese population.