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目的观察两种丝裂原蛋白激酶(mitogen activated protein kinases,MAPK)抑制剂PD98059及U0126对刚地弓形虫侵入宿主细胞的影响,探讨其对弓形虫速殖子侵入宿主细胞信号转导途径的不同阻断效应。方法丝裂原蛋白激酶抑制剂PD98059或U0126分别在不同时间及不同剂量作用于速殖子-宿主细胞培养系统,用流式细胞仪(FCM)检测宿主细胞感染速殖子的差异。结果流式细胞仪检测加入U01261μmol/L、10μmol/L和100μmol/L的细胞培养孔的细胞感染弓形虫速殖子的量分别比对照组平均降低了26.10%(P<0.01),66.42%(P<0.01)和70.39%(P<0.01)。而加入PD980591μmol/L、10μmol/L和100μmol/L的分别比对照组平均降低了25.45%(P<0.01),53.01%(P<0.01)和64.70%(P<0.01)。实验中发现100μmol/L U0126作用培养细胞9h的时候,可导致HL-60细胞出现部分聚集成团、漂浮的中毒现象。结论U0126和PD98059均可明显抑制弓形虫速殖子侵入宿主细胞,但其差异无显著性,其机制有待进一步探索。
Objective To investigate the effects of two mitogen-activated protein kinase (MAPK) inhibitors, PD98059 and U0126, on invasion of Toxoplasma gondii into host cells and their differences in the signal transduction pathways of Toxoplasma gondii invaded into host cells Blocking effect. Methods The mitogen-derived protein kinase inhibitor PD98059 or U0126 was used in tachyzoite-host cell culture system at different time and different doses, and the difference of host cell infected tachyzoites was detected by flow cytometry (FCM). Results The results showed that the amount of Toxoplasma gondii tachyzoites infected with U01261μmol / L, 10μmol / L and 100μmol / L cells were reduced by 26.10% (P <0.01) and 66.42% (P < P <0.01) and 70.39% (P <0.01). However, the addition of PD980591μmol / L, 10μmol / L and 100μmol / L decreased by 25.45% (P <0.01), 53.01% (P <0.01) and 64.70% (P <0.01) on average. The experiment found that when U0126 cells were treated with 100μmol / L U0126 for 9h, HL-60 cells could be partially aggregated and floated. Conclusion Both U0126 and PD98059 can significantly inhibit Toxoplasma gondii invasion of host cells, but the difference was not significant, the mechanism needs further exploration.