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研究了电子束辐照降低花生过敏原免疫原性的效果及辐照对花生生化性质的影响。选择2、4、6、8、10、15和20kGy剂量的电子束对花生过敏原液、脱脂花生粉进行辐照处理,辐照后花生主要过敏原(Ara h 1,Ara h 2,Ara h 3)蛋白分子质量的变化通过SDS-聚丙烯酰胺凝胶电泳测定,免疫原性的变化通过免疫印迹和间接竞争ELISA法测定;辐照后花生过敏原溶液的浓度、浊度及疏水性的变化通过紫外分光光度计和荧光光度计分别进行测定。结果表明,花生过敏原在溶液状态比固体状态对辐照更为敏感。当辐照剂量低于10kGy时处理的过敏原液的免疫原性稍有增强,剂量大于10kGy时免疫原性降低,剂量为20kGy时过敏原液的IC50值是未处理的11倍。辐照使过敏液的浓度和浊度随着剂量的加大而增加,疏水性与对照组相比增强,但当剂量大于15kGy时降低。电子束辐照改变了过敏原的生化性质,降低了其免疫原性,比较而言,辐照对液体状态下过敏原的影响更显著。
The effects of electron beam irradiation on reducing the immunogenicity of peanut allergens and the effects of irradiation on the biochemical properties of peanut were studied. Peanut allergens and defatted peanut powder were irradiated by electron beam at doses of 2, 4, 6, 8, 10, 15, and 20kGy, and the main peanut allergens (Arah1, Arah2, Arah3 ) The change of the molecular weight of the protein was determined by SDS-polyacrylamide gel electrophoresis and the change of immunogenicity was determined by immunoblotting and indirect competitive ELISA. The change of the concentration, turbidity and hydrophobicity of the solution of peanut allergen after irradiation UV spectrophotometer and fluorescence photometer were measured. The results showed that peanut allergens were more sensitive to irradiation than solid state in solution. The immunogenicity of the allergen solution treated slightly increased at doses less than 10 kGy and decreased at doses greater than 10 kGy with an IC50 of 11 times that of the untreated solution at a dose of 20 kGy. Irradiation increased the concentration and turbidity of the allergy solution with increasing dose, and the hydrophobicity increased compared with the control group, but decreased when the dose was greater than 15 kGy. Electron beam irradiation changed the biochemical properties of allergens and decreased their immunogenicity. In contrast, the effect of irradiation on allergens in liquid state was more pronounced.