乳酸脱氢酶反应器用于流动注射分析测定人体液中的L-乳酸

• 作 者: 唐庆 胡冠九 李齐芳 都恒华
• 机 构: 南京大学化学系,南京大学化学系,南京大学化学系,南京大学化学系 南京 210008,南京 210008,南京 210008,南京 210008
• 出 处: 分析化学
• 发表日期: 1992年12期
• 关键词: 流动注射分析 三乙氧基硅烷 米氏常数 氨丙基 胺化 载液 二次重蒸馏水 参比溶液 空白值 吸光度

本文用[3-(2-氨乙基)氨丙基]三乙氧基硅烷试剂胺化一定孔径大小的载体,用戊二醛作偶联剂,将乳酸脱氢酶固定于其上,装入柱内,联结于流动体系中。L-乳酸的浓度在0.1～4.4mmol/L呈线性,检测限为0.4nmol(s/N=4),相对标准偏差≤1.2％,回收率在96％～103％。每小时进样35次。测定了固定化LDH的表观米氏常数及人血清和尿中的L-乳酸。

In this paper, [3- (2-aminoethyl) aminopropyl] triethoxysilane reagent was used to aminate a carrier with a certain pore size, and glutaraldehyde was used as a coupling agent to immobilize lactate dehydrogenase Into the column, connected to the flow system. The concentration of L-lactic acid was linear in the range of 0.1-4.4 mmol / L, the detection limit was 0.4 nmol (s / N = 4), the relative standard deviation was less than 1.2% and the recovery was 96% -103%. Injection 35 times per hour. The apparent Michaelis constant of immobilized LDH and L-lactic acid in human serum and urine were measured.