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微量血体外培养后制备染色体标本,作为细胞遗传学效应指标,已在临床医学、工业卫生、遗传毒理学等方面广泛采用。但在进行环境诱变剂(如工业毒物、农药、药物等)所致潜在性危害的研究中,常用的染色体畸变分析方法由于操作繁复、工作量大、需经专门训练等原因而远远不相适应,因而迫切需要寻找操作简易、快速的测定方法。我室参照了国内外报道的有关外周血淋巴细胞和骨髓多染红细胞微核测定法,进行了苯的诱变作用实验研究,取得了与染色体畸变一致的结果,并已初步用于放射损伤及化学诱变的临床观察。现将方法介绍于后。
Chromosome samples prepared after culture in vitro, as a cytogenetic effect indicators, has been widely used in clinical medicine, industrial hygiene, genotoxicity and other aspects. However, in the study of the potential hazards caused by environmental mutagens (such as industrial poisons, pesticides, drugs, etc.), the commonly used methods of chromosome aberration analysis are far from being complicated due to complicated operation, heavy workload and special training Therefore, there is an urgent need to find a simple and rapid method of determination. My room with reference to the reports of peripheral blood lymphocytes and bone marrow polychromatic erythrocytes micronucleus assay, the experimental study of benzene mutagenicity, obtained consistent with chromosomal aberrations results and has been initially used for radiation injury and Clinical Observation of Chemical Mutagenesis. The method is introduced later.