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Three main parameters were selected to study their importance in transformation by budmicroinjection in non-head Chinese cabbage [Brassica campestris ssp. chinensis (L.) Makinovar. communis Tsen et Lee]. The results showed that the developmental stage of floral bud, theconcentrations of sucrose and surfactant Silwet L-77 were critical for the successfultransformation by this new method. The suitable bud size is 2-3 mm in length, the favorableconcentration of sucrose and surfactant Silwet L-77 are 8 and 0.02% respectively. When thesucrose concentration was greater than 10% or that of Silwet L-77 was above 0.1%, the treatedbuds became yellow and finally blighted. 4/6 T1 seedlings resistant to kanamycin were positiveby PCR analysis, and T2 progeny of all these positive T1 plants have one or more hybridizingbands by Southern blot. Under 5% sucrose, 0.02% Silwet L-77 and grade 2 bud (2-3 mm in itslength) parameters, the most favorable transformation efficiency is about 0.56%, and meanefficiency reaches 0.16% in all experiments indicating that bud microinjection is potentialtransformation way in non-head Chinese cabbage.
Three main parameters were selected to study their importance in transformation by budmicroinjection in non-head Chinese cabbage [Brassica campestris ssp. Chinensis (L.) Makinovar. Communis Tsen et Lee]. The results showed that the developmental stage of floral bud, theconcentrations of sucrose and surfactant Silwet L-77 were critical for the success rate of this new method. When the sucrose concentration was 2-3 mm in length, the favorable concentration of sucrose and surfactant Silwet L-77 were 8 and 0.02% respectively. greater than 10% or that of Silwet L-77 was above 0.1%, the treated buds became yellow and finally blighted. 4/6 T1 seedlings resistant to kanamycin were positive by PCR analysis, and T2 progeny of all these positive T1 plants have one or more hybridizing bands by Southern blot. Under 5% sucrose, 0.02% Silwet L-77 and grade 2 bud (2-3 mm in its length) parameters, the most favorable transformation efficiency is about 0.56%, and meaneffici ency reaches 0.16% in all experiments indicating that bud microjection is potential transformation way in non-head Chinese cabbage.