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目的:建立心血宁胶囊中葛根素的HPLC含量测定方法。方法:色谱柱:以十八烷基硅烷键合硅胶为填充剂,流动相为:甲醇∶水∶醋酸(25∶75∶0.2)。流速:1.0?mL/min。检测波长:250?nm,柱温:40?℃。结果:葛根素在0.1674~0.7254?μg具良好的线性关系(r=0.999?7)??;平均回收率为97.7%,RSD=1.5%(n=5)。结论:该方法简便,准确,重复性好,可用于心血宁胶囊中葛根素的含量测定。
Objective: To establish HPLC method for the determination of puerarin in “Xuexuening capsule”. Methods: Column: octadecylsilane bonded silica as a filler, mobile phase: methanol: water: acetic acid (25:75:0.2). Flow rate: 1.0? ML / min. Detection wavelength: 250? Nm, column temperature: 40? ℃. Results: There was a good linear relationship between puerarin and 0.1674 ~ 0.7254 μg (r = 0.999 7). The average recovery was 97.7% and RSD was 1.5% (n = 5). Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of puerarin in Xuxuening capsule.