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目的 建立阿尔茨海默症转基因动物模型 ,为病因研究和药物筛选提供了一个合适的动物模型。 方法 通过显微注射方法将外源 DNA注射到小鼠受精卵的雄性原核 ,移植到假孕母鼠的输卵管。仔鼠出生后 ,经PCR及 PCR- Southern杂交检测阳性小鼠。 结果 共注射 887枚受精卵 ,存活 482枚 ,移卵后产仔 35只 ,阳性 3只。阳性鼠分别传代 ,在 F2代获得纯合子后建系。免疫组织化学显示在大脑皮层、小脑及海马的神经细胞有 Aβ沉淀形成。 结论 通过显微注射的方法已建立与人类发病机理相似的阿尔茨海默症转基因动物模型。
Objective To establish a transgenic animal model of Alzheimer’s disease and to provide a suitable animal model for etiological research and drug screening. Methods Exogenous DNA was injected into the male pronucleus of mouse fertilized eggs by microinjection and transplanted into the fallopian tubes of pseudopregnant female rats. After birth, the positive mice were detected by PCR and PCR-Southern blot. Results A total of 887 fertilized eggs were injected and 482 survived. 35 were born after oviposition and 3 were positive. Positive mice were passaged, respectively, in the F2 generation of homozygotes after the Department. Immunohistochemistry showed that neurons in the cerebral cortex, cerebellum and hippocampus had Aβ precipitate formation. Conclusion Alzheimer’s disease transgenic animal model has been established by microinjection.