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目的为鉴定单纯疱疹病毒(HSV)糖蛋白C(gC)一个短肽模拟表位mgC基因作为HSVDNA表位疫苗的可能性 ,需构建含此表位的真核载体。方法合成编码该表位的单链DNA ,经不对称PCR扩增后 ,克隆入真核表达载体pcD NA3.1中 ,并在CHO细胞中表达。用RT PCR法鉴定mRNA的表达。结果含mgC基因的真核表达载体在哺乳动物细胞中可以在mRNA水平表达外源蛋白。结论成功地构建了HSVgC中短肽模拟表位mgC基因的真核表达载体 ,为进一步探讨该HSV模拟表位的功能奠定了基础。
OBJECTIVE: To identify the possibility of using mgC gene, a short peptide mimic epitope of herpes simplex virus (HSV) glycoprotein C (gC), as an epitope vaccine for HSVDNA, a eukaryotic vector containing this epitope needs to be constructed. Methods The single-stranded DNA encoding this epitope was synthesized and amplified by asymmetric PCR. The recombinant plasmid was cloned into the eukaryotic expression vector pcD NA3.1 and expressed in CHO cells. The expression of mRNA was identified by RT PCR. Results The eukaryotic expression vector containing the mgC gene can express foreign proteins at the mRNA level in mammalian cells. Conclusion The eukaryotic expression vector of mgC gene, a short peptide mimic epitope of HSVgC, was successfully constructed, which lays the foundation for further exploring the function of HSVgC mimotope.