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目的了解石家庄市肉鸡屠宰和市售环节中弯曲菌的污染状况及对12种抗生素的耐药谱和耐药特征。方法采集屠宰前肉鸡肛拭子、鸡酮体涂抹拭子标本和市售鸡肉、鸡肝脏样品,参照GB 4789.9—2014《食品安全国家标准食品微生物学检验空肠弯曲菌检验》和多重聚合酶链式反应(PCR)方法进行弯曲菌的分离和鉴定,用K-B纸片法对分离弯曲菌进行6类12种抗生素药敏测定。结果在439份标本/样品中共有17份检出弯曲菌,总检出率为3.9%(17/439)。空肠弯曲菌对萘啶酸、左氧氟沙星、环丙沙星和四环素的耐药率均为85.7%(6/7),对庆大霉素、链霉素、丁胺卡那、阿奇霉素、氟苯尼考全部敏感。结肠弯曲菌除对阿奇霉素、多西环素、氟苯尼考的耐药率小于50.0%外,对环丙沙星、左氧氟沙星、萘啶酸、庆大霉素、丁胺卡那、四环素、链霉素、克林霉素的耐药率均高于80.0%。17株弯曲菌多重耐药率为70.6%(12/17),呈11种耐药谱型,市售环节分离株耐药谱型多于屠宰环节分离株,并具有各自不同的优势耐药谱型。结论石家庄市肉鸡屠宰和市售环节均有一定程度的弯曲菌污染,分离株多重耐药情况严重,呈多种耐药谱型。
Objective To understand the status of Campylobacter spp. In slaughter and commercial segments of Shijiazhuang broiler chickens and its resistance spectrum and resistance to 12 antibiotics. Methods Pre-slaughter chicken anal swabs, chicken ketone body smear swab specimens and commercially available chicken, chicken liver samples, with reference to GB 4789.9-2014 “National Food Safety Standard Microbiological Test of Campylobacter jejuni” and multiple polymerase chain Reaction (PCR) method for the separation and identification of Campylobacter, using KB paper method for the separation of Campylobacter 6 kinds of 12 antibiotic susceptibility testing. Results A total of 17 Campylobacter species were detected in 439 samples / samples with a total detection rate of 3.9% (17/439). The resistance rates of Campylobacter jejuni to nalidixic acid, levofloxacin, ciprofloxacin and tetracycline were all 85.7% (6/7). The resistance to gentamicin, streptomycin, amikacin, azithromycin, Test all sensitive. In addition to the azithromycin, doxycycline, florfenicol resistance rate of less than 50.0%, Campylobacter coli, ciprofloxacin, levofloxacin, nalidixic acid, gentamicin, butylamine, tetracycline, the chain The rates of resistance to clindamycin and clindamycin were higher than 80.0%. The multi-drug resistance rate of 17 strains of Campylobacter was 70.6% (12/17), showing eleven kinds of drug resistance spectrum. The drug resistance spectrum of commercially available segment isolates was more than that of slaughter part isolates, and had different predominance drug resistance spectrum type. Conclusion There was some degree of Campylobacter contamination in the slaughtering and marketing segments of broiler chickens in Shijiazhuang. The multi-drug resistant strains of the isolates were serious and showed multiple drug resistance patterns.