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目的观察姜黄素预处理对慢性应激大鼠行为学、单胺类递质水平及血清糖皮质激素的影响,为评价姜黄素对慢性心理应激大鼠中枢神经的保护作用提供依据。方法 48只Wistar大鼠随机分为对照组、慢性应激组、氟西汀干预组、姜黄素(低、中、高剂量)干预组,每天应激前1 h灌胃给药。对大鼠进行28 d应激刺激建立慢性不可预知性慢性应激模型。旷场试验测定大鼠行为学指标;糖水试验测定大鼠“快感缺乏”;高效液相-电化学检测法测定海马单胺类递质;ELISA检测血清糖皮质激素。结果慢性应激组大鼠的体质量、旷场活动性和糖水偏嗜度均明显低于对照组(P<0.05,P<0.01);海马5-HT含量下降(P<0.05),血清糖皮质激素水平升高(P<0.01)。3个剂量姜黄素组大鼠体质量于第4周分别为(310.60±21.35)、(315.60±23.73)和(319.91±18.76)g,高于慢性应激组〔(270.24±19.60)g,P<0.05〕;旷场竖立时间分别为(18.86±9.49)、(24.14±4.38)和(24.14±5.41)s,高于慢性应激组〔(11.00±9.20)s,P<0.05〕;血清糖皮质激素水平分别为(567.94±22.54)、(338.62±63.79)和(259.96±17.79)ng/L,低于慢性应激组〔(908.57±22.86)ng/L,P<0.01〕。实验后低、中剂量组海马5-HT含量分别为(1063.89±149.61)和(1340.96±42.62)ng/g,高于慢性应激组〔(941.23±80.00)ng/g,P<0.05〕。结论姜黄素预处理可改善慢性不可预知性应激大鼠抑郁状态,起到中枢神经保护作用,其作用机制可能与单胺类递质水平的增加和调节HPA轴活性有关。
Objective To observe the effect of curcumin preconditioning on behavior, monoamine neurotransmitter and serum glucocorticoid in chronic stress rats, and to provide evidence for evaluating the protective effect of curcumin on the central nervous system in chronic psychological stress rats. Methods 48 Wistar rats were randomly divided into control group, chronic stress group, fluoxetine intervention group and curcumin (low, medium and high dose) intervention groups. Rats were subjected to 28 d stress stimulation to establish a chronic unpredictable chronic stress model. The open field test was used to determine the behavioral indexes of rats. The rats were tested for “lack of pleasure” in sugar water test, monoamine neurotransmitters in hippocampus by high performance liquid chromatography-electrochemical detection, and glucocorticoid in serum. Results The body mass, open-field activity and degree of glycosidation in chronic stress group were significantly lower than those in control group (P <0.05, P <0.01). The content of 5-HT in hippocampus was decreased Corticosteroid levels increased (P <0.01). The body weight of the three doses of curcumin group were (310.60 ± 21.35), (315.60 ± 23.73) and (319.91 ± 18.76) g respectively, higher than that of the chronic stress group [(270.24 ± 19.60) g, P <0.05]. The open time in the open field was (18.86 ± 9.49), (24.14 ± 4.38) and (24.14 ± 5.41) s respectively, higher than that in the chronic stress group [(11.00 ± 9.20) s, P < The level of corticosteroids were (567.94 ± 22.54), (338.62 ± 63.79) and (259.96 ± 17.79) ng / L, respectively, lower than the chronic stress group [(908.57 ± 22.86) ng / L, P <0.01〕. The levels of 5-HT in the hippocampus of the low and middle dose groups after the experiment were (1063.89 ± 149.61) and (1340.96 ± 42.62) ng / g, respectively, higher than that of the chronic stress group [(941.23 ± 80.00) ng / g, P <0.05]. CONCLUSION: Curcumin preconditioning can improve the depressive state of rats with chronic unpredictable stress and play a central neuroprotective role. The mechanism may be related to the increase of monoamine neurotransmitters and the regulation of HPA axis activity.