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目的:了解宁波地区不同来源副溶血性弧菌的毒力及血清型、基因型,为追溯病原和防控副溶血性弧菌提供依据。方法:海产品菌株分离采用PCR+GB筛检方法,病人采用全国临床检验操作规程方法;用诊断血清进行血清学分型,用PFGE进行基因分型;用PCR检测tdh和trh毒力基因。结果:海产品流行优势血清群为O6群和O5群,病人株为O3群;海产品株PFGE分型与病人株相比较为分散;88.04%的病人株带毒力基因,海产品株带毒力基因仅有1.11%。结论:海产品株和病人株在流行优势株上存在明显差别,提示血清型分型和PFGE分型能发现菌株间的亲缘关系,可用于病原的追踪与溯源;病人株带毒率明显高于海产品株(P<0.01),认为海产品株致病性不强,建议将VP分为致病的和非致病两类,有利于鉴别和防控措施的改进。
Objective: To understand the virulence and serotypes and genotypes of Vibrio parahaemolyticus from different sources in Ningbo, and to provide basis for tracing the pathogen and preventing and controlling Vibrio parahaemolyticus. Methods: The strains of marine products were isolated by PCR + GB screening method. The patients adopted the national clinical testing procedures and methods. The serotypes of the strains were screened with the diagnostic serum and genotyped by PFGE. The virulence genes of tdh and trh were detected by PCR. Results: The predominant serogroups of seafood were O6 group and O5 group, and the patient strain was O3 group. The PFGE classification of seafood strains was more dispersed than that of the patient strains. 88.04% of the strains were virulence genes, Gene of force is only 1.11%. Conclusion: There is a significant difference between the strains of predominant strains and the strains of endemic strains. It suggests that the serotyping and PFGE typing can find the genetic relationship among isolates and can be used for tracing and tracing pathogens. The virulence of patients is obviously higher than that of the sea Product strains (P <0.01), that the pathogenicity of seafood strains is not strong, VP is suggested to be divided into pathogenic and non-pathogenic two categories, is conducive to identification and prevention and control measures to improve.