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本研究利用特异引物对来源于云南的22份栽培稻及分别来源于海南、云南元江、江西东乡的3份普通野生稻(O.rufipogon Griff.)和1份长雄野生稻(O.longistaminata)Wx基因中第1内含子和第1内含子上游微卫星序列(CT)n的序列进行PCR扩增并测序。结果显示所有材料均能扩增出产物并获得碱基测序(包括长雄野生稻),4份野生稻的(CT)重复次数分别是(CT)8、(CT)10、(CT)10、(CT)11,较栽培稻少;第1内含子中+1位碱基均为G。相较于栽培稻,野生稻在第1内含子与(CT)n重复中有明显区别于栽培稻的SNP位点,包含基因突变的In Del、转换和颠换3种类型。说明野生稻和栽培稻在进化的过程中出现了差异。Wx基因中微卫星序列(CT)n和第1内含子的序列可作为检测野生稻遗传组分的一个遗传标签。
In this study, 22 cultivated rice from Yunnan and 3 wild O.rufipogon Griff. And one longan O. longissima from Hainan Province, Yuanjiang of Yunnan Province, Dongxiang of Jiangxi Province, The sequence of the first intron and the upstream of the first intron in Wx gene was amplified by PCR and sequenced. (CT) 8, (CT) 10, (CT) 10, and (CT) 10 were obtained from all the materials. (CT) 11, less than cultivated rice; the first intron of the +1 base are G. Compared with cultivated rice, wild rice had obvious difference from the SNP locus in intron 1 and (CT) n repeats, including In Del, gene mutation and transversion. The difference between wild rice and cultivated rice in the evolutionary process is illustrated. The sequence of microsatellite sequence (CT) n and the first intron of Wx gene can be used as a genetic marker to detect the genetic components of wild rice.