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研究采用胚轴切断扦插法诱导棉株产生抗虫性。该方法是在棉花２片真叶期，将其根茎在下胚轴处切断，接种假单胞菌，扦插使其生根。通过该机械损伤及菌处理，诱导棉株产生系统抗性。实验设菌诱导扦插株和无菌诱导扦插株两项处理，以自然生长棉株作对照。诱导棉株扦插时，接种假单胞菌（ＰｓｅｕｄｏｍｏｎａｇｌａｄｉｏｌｉＤ－２２５１）的抗利福平突变菌株。生根后取其根、茎、叶组织，检测其体内接种菌菌量，结果发现诱导菌能够在棉株体内定殖，其浓度高低顺序为：根＞茎＞叶；诱导后，棉株体内单宁含量在各生长期均有不同程度提高，菌诱导株和无菌诱导株的变幅分别为３０．７８％～２８９．９９％和３．８８％～１３９．６９％。除盛花期外，各生长期酚含量也有所提高，菌诱导株和无菌诱导株的变幅分别为２．６０％～３６．２２％和１．３２％～２６．１６％；温室条件下，对诱导株上的自然感蚜状况进行了调查；５叶期，菌诱导和无菌诱导棉株的百株蚜量分别降低７４．９５％和４３．５１％，有蚜株率分别降低６７．８４％和５４．９８％；７叶期，百株蚜量分别降低３５．９８％和１７．２１％，有蚜株率分别降低３２．２４％和１７．２１％。
In this study, hypocotyl cuttings were used to induce cotton plants to develop insect resistance. The method is that two true leaves of cotton are cut off at the hypocotyls of rhizomes, inoculated with Pseudomonas, and cuttings are made to root. Through this mechanical damage and bacterial treatment, cotton plants were induced to develop systemic resistance. Experimental set bacteria-induced cuttings and sterile-induced cuttings two treatment, with the natural growth of cotton as a control. Ripening mutants of Pseudomonas sp. D-2251 were inoculated with the cotton plant cuttings. The roots, stems and leaves were taken from the roots of the cotton plants and the amount of bacteria inoculated in them was detected. The results showed that the inducible bacteria could colonize the cotton plants in the order of root> stem> leaf. After inoculation, The content of Ning in different growth stages increased to some extent. The amplitude of the induced strain and the sterile induced strain were 30.78% -289.99% and 3.88% -139.69%, respectively. In addition to the flowering stage, the content of phenols in each growth phase also increased, the amplitude of the induced and sterile strains were 2.60% -36.22% and 1.32% -26.16% respectively. Under the greenhouse conditions , And the natural aphids on the induced plants were investigated. In the 5-leaf stage, the aphid population of one hundred plants decreased by 74.95% and 43.51%, respectively, with aphid strain reduced by 67 .84% and 54.98%, respectively. The number of aphids decreased by 35.98% and 17.21% respectively in the seven-leaf stage and in the one-hundred-year-old aphids. The rates of aphids were reduced by 32.24% and 17.21%, respectively.