15例急性淋巴细胞性白血病微小残留病追踪检测结果分析

• 作 者: 肖燕 梁欣荃 费洪宝
• 机 构: 同济医科大学附属协和医院儿科,同济医科大学附属协和医院儿科,同济医科大学附属协和医院儿科 430022,430022,430022
• 出 处: 临床儿科杂志
• 发表日期: 1999年05期
• 关键词: 微小残留病 白血病细胞 基因重排 重排基因 转录本 杂交法 白血病患儿 诱导缓解 急淋 抗原受体

为探讨残留白血病细胞的存在与白血病患儿长期生存之间的关系,我们用非同位素的PCR-RNA转录本分子杂交法,选用T细胞抗原受体γ链(TCR_γ)重排基因为标志,检测了20例急性淋巴细胞白血病(ALL)患儿,并对其中具有TCR_γ重排基因的15例患儿进行了追踪检测,结果显示:化疗后早期(<5月)大多数患儿都能检测到残留白血病细胞,长期缓解者微小残留病检测为阴性。结果表明,当临床达到完全缓解时,对每1例患者的残留白血病细胞进行监测是必要的。

To explore the relationship between the presence of residual leukemia cells and the long-term survival of children with leukemia, we used a non-isotopic PCR-RNA transcript hybridization method, using the T cell antigen receptor γ chain (TCR_γ) rearrangement gene as a marker to detect Twenty patients with acute lymphoblastic leukemia (ALL) and 15 children with TCR_γ rearrangement genes were followed. The results showed that most children in the early (<5 months) period after chemotherapy were detected. Residual leukemia cells, long-term remission minor residual disease test was negative. The results showed that it was necessary to monitor the residual leukemia cells in each patient when the clinical response to complete remission was achieved.