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目的:观察溃结灵含药血清对结肠上皮细胞损伤模型MUC-2及TGF-α蛋白含量的影响,进一步探讨溃结灵促进肠黏膜损伤修复的机理。方法:TNF-α(50ng/mL)干预大鼠结肠上皮细胞6h造成细胞损伤模型,采用Elisa方法检测不同浓度溃结灵含药血清对细胞损伤模型MUC-2及TGF-α蛋白含量的影响。结果:①结肠上皮细胞损伤模型组MUC-2蛋白含量较空白对照组升高(P<0.05);②溃结灵含药血清高剂量组MUC-2蛋白含量较模型对照组升高(P<0.05);溃结灵含药血清高剂量组TGF-α蛋白含量较模型对照组升高(P<0.01)。结论:溃结灵含药血清可促进TNF-α致结肠上皮细胞损伤模型中MUC-2、TGF-α含量升高,进而促进受损肠黏膜上皮的修复。
OBJECTIVE: To observe the effect of Kuijieling serum on the protein content of MUC-2 and TGF-α in colonic epithelial cell injury model and further explore the mechanism of Kuijieling promoting intestinal mucosal injury repair. Methods: TNF-α (50ng / mL) was used to induce cell injury in rat colon epithelial cells for 6h. Elisa method was used to detect the effect of different concentrations of Jiezhiling serum on the protein levels of MUC-2 and TGF-α in cell injury model. Results: ①The content of MUC-2 protein in colonic epithelial cell injury model group was higher than that of blank control group (P <0.05); ② The content of MUC-2 protein in high dosage of Jiejiling capsule group was higher than that of model control group (P < 0.05). The protein content of TGF-α in high dosage of Kuijieling serum was higher than that in model control group (P <0.01). Conclusion: Kuijieling serum can promote the TNF-α induced model of colonic epithelial injury in MUC-2, TGF-α content increased, thereby promoting the repair of damaged intestinal mucosa epithelium.