论文部分内容阅读
采用无皂乳液聚合法制得聚苯乙烯-甲基丙烯酸缩水甘油酯(PSG)乳液微球,然后在微球表面嫁接空间臂分子1,6-己二胺,得到表面含氨基的PSGN微球,接着借助EDC/NHS催化作用将药物分子卡托普利化学偶联到PSGN微球表面,制成固定卡托普利的亲和PSG微球。实验着重考察了PSGN微球偶联固定卡托普利反应过程中催化剂比例和用量、pH值、反应温度和时间等的影响规律。结果表明,在25℃,pH为4.0,m(NHS)∶m(EDC)=1∶2,EDC的浓度为4mg/mL的条件下,卡托普利偶联到微球表面的效果较好。
PSG emulsion microspheres were prepared by soap-free emulsion polymerization, then the space arm molecule, hexamethylenediamine, was grafted on the surface of the microspheres to obtain PSGN microspheres with amino groups on the surface. Subsequently, the drug molecule Captopril was chemically coupled to the surface of the PSGN microspheres by means of EDC / NHS catalysis to prepare affinity captopril affinity PSG microspheres. The experiment focused on the effects of the ratio and amount of catalyst, pH value, reaction temperature and time on the immobilization of captopril in PSGN microspheres. The results showed that captopril was more effective on the surface of microspheres under the conditions of 25 ℃, pH 4.0, m (NHS): m (EDC) = 1: 2 and EDC concentration of 4mg / mL .