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以地高辛标记的前生长抑素原ｃＲＮＡ（Ｐ－Ｐ－ＳＳｃＲＮＡ）探针，用原位杂交技术研究了大鼠大脑皮质ＳＳｍＲＮＡ神经元的生后早期发育，结果显示：１．旧皮质中的梨状皮质，以扣带回皮质为代表的中间皮质和新皮质中均存在ＳＳｍＲＮＡ神经元。２．ＳＳｍＲＮＡ神经元主要位于梨状皮质深层，从生后第１ｄ（Ｐｌ）至生后第５ｄ（Ｐ５）阳性细胞数增多，体积变大，至生后第１０ｄ（Ｐｌ０）细胞数有减少的趋向。３．扣带回皮质的ＳＳ基因表达较为恒定，从Ｐｌ至Ｐｌ０，阳性细胞较均匀地分布于除第１层以外的各层。４．新皮质ＳＳｍＲＮＡ神经元呈特定的板层状分布，生后第１ｄ主要定位于颗粒下层（Ⅴ、Ⅵ层），至Ｐ５～Ｐｌ０，除仍占据颗粒下层外，颗粒上层亦出现较多淡染的阳性细胞。５，在个别大鼠，于生后第１ｄ的新皮质中见到许多特殊阳性束状结构，该束的深部夹杂一些ＳＳｍＲＮＡ神经元。有的细胞突起与束平行，似有沿束向皮质表面迁移的趋势。上述结果表明，出自同一前体原的ＳＳ１４，ＳＳ２８，ＳＳ２８（１～１２）可能均参与鼠脑早期发育的调节。
Using digoxigenin-labeled pro-somatostatin cRNA (P-P-SScRNA) probe, in situ hybridization was used to study the early postnatal development of SSmRNA neurons in rat cerebral cortex. The results showed: 1. There are SSmRNA neurons in the piriform cortex in the old cortex and in the cortex and the neocortex, which are represented by the cingulate cortex. 2. The majority of SSmRNA neurons were located in the deep layer of piriform cortex. The number of positive cells increased from the first day of life (P1) to the fifth day of life (P5), the volume of SSmRNA neurons increased, and the number of cells decreased to the tenth day after birth (P10). 3. The expression of SS gene in cingulate cortex was relatively constant. From Pl to Pl0, the positive cells were distributed more evenly than the first layer. 4. The neocortical SSmRNA neurons showed a lamellar distribution. At 1 day after birth, they were mainly located in the lower layer (Ⅴ and Ⅵ), and from P5 to Pl0, except those that still occupied the lower layer of the granule, more light-stained Positive cells. In individual rats, many special positive bundle structures were found in the neocortex on the first day after birth, and some SSmRNA neurons were involved in the deep part of the bundle. Some cell protrusions parallel with the beam, it seems that there is a tendency to migrate to the cortical surface. The above results indicate that SS14, SS28, SS28 (1 ~ 12) from the same progenitor may participate in the regulation of early development of rat brain.