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建立快速灵敏特异的甲肝减毒活疫苗滴度的ＲＴ－ＰＣＲ检测法。方法：以编码甲肝病毒（ＨＡＶ）ＶＰ３羧基端的基因区为目标区，运用ＲＴ－ＰＣＲ法对甲肝疫苗病毒进行检测，并与ＴＣＩＤ５０检测法进行比较。结果：ＲＴ－ＰＣＲ法灵敏度和特异性均与ＴＣＩＤ５０法相似，...
To establish a rapid and sensitive specific hepatitis A attenuated live vaccine titer RT-PCR assay. Methods: The gene region coding for the carboxy terminal region of VP3 of hepatitis A virus (HAV) was selected as the target region. The hepatitis A vaccine virus was detected by RT-PCR and compared with TCID50. Results: RT-PCR method sensitivity and specificity are similar with the TCID50 method, ...