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目的 大肠癌预后取决于是否存在肿瘤转移 ,目前尚无肯定的观测大肠癌细胞恶性潜能的生物学指标。本研究目的在于探讨nm2 3 H1作为预测大肠癌转移指标的潜在价值。方法 采用免疫组织化学链霉菌亲合物素蛋白—生物素酶标 (SP)方法对 5 8例存档大肠癌标本进行nm2 3 H1蛋白表达检测 ,用SSPS统计软件包对nm H1表达与临床病理参数的关系进行分析。结果 正常大肠粘膜和大肠腺瘤nm2 3呈阳性表达 ,6 2 1%大肠癌组织阳性表达。nm2 3表达与肿瘤分级、淋巴结及肝转移负相关 (P值分别为 0 0 10 0 ,0 2 0 87,0 0 0 376 )。nm2 3阳性表达的大肠癌患者预后好(P =0 0 0 0 2 )。结论 nm2 3 H1是预测大肠癌细胞转移潜能和预后的敏感指标
The purpose of the prognosis of colorectal cancer depends on the existence of tumor metastasis, there is no certain biological indicators for the observation of malignant potential of colorectal cancer cells. The purpose of this study was to investigate the potential value of nm23 H1 as a predictor of metastasis in colorectal cancer. Methods Immunohistochemical streptavidin-biotinylated enzyme-linked immunosorbent assay (SP method) was used to detect the expression of nm23 H1 protein in 58 specimens of colorectal cancer. The expression of nm H1 and the clinicopathological parameters The relationship between the analysis. Results The positive expression of nm23 in colorectal mucosa and colorectal adenoma was positive and 62.1% in colorectal carcinoma. nm23 expression was negatively correlated with tumor grade, lymph node and liver metastasis (P values were 0 0 10 0, 0 2 0 87, 0 0 0 376, respectively). Patients with colorectal cancer with nm23 positive expression had a good prognosis (P = 0.00002). Conclusion nm23 H1 is a sensitive index for predicting the metastatic potential and prognosis of colorectal cancer cells